Journal: iScience

Article Title: Prognostic value of BTG1 for predicting decitabine sensitivity in de novo acute myeloid leukemia

doi: 10.1016/j.isci.2025.114327

Figure Lengend Snippet: Downstream signaling pathway of BTG1 in AML cell lines (A) Top three signaling pathways in terms of Gene Ratio were EBV infection (0.037), Wnt signaling pathway (0.035), and hematopoietic lineage (0.023) of KEGG pathway enrichment. (B) Effect of BTG1 on the mRNA expression of β-catenin, Cyclin D1, and C-Myc. (C) Effect of BTG1 on the protein expression of β-catenin and Cyclin D1. (D) The relative viability of AML cells after interference of BTG1 and treatment with FH535. (E) Apoptosis of AML cells after interference of BTG1 and treatment with FH535. Data are presented as mean ± standard deviation (SD). (ns, p > 0.05; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001).

Article Snippet: Mouse monoclonal anti-human Cyclin D1 , Proteintech , Cat# 60186-1-Ig RRID: AB_10793718.

Techniques: Protein-Protein interactions, Infection, Expressing, Standard Deviation

Journal: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

Article Title: MicroRNA-623 Targets Cyclin D1 to Inhibit Cell Proliferation and Enhance the Chemosensitivity of Cells to 5-Fluorouracil in Gastric Cancer

doi: 10.3727/096504018x15193469240508

Figure Lengend Snippet: Figure 3. miR-623 directly targets cyclin D1 (CCND1) in GC. (A) Wild-type (WT) and mutated (MUT) miR-623 binding sequences in the 3¢-untranslated region (3¢-UTR) of CCND1. (B) RT-qPCR and (C) Western blot were performed to determine the mRNA and protein levels of CCND1 in SGC-7901 and BGC-823 cells transfected with miR-623 mimic or miR-NC. *p < 0.05 compared with miR-NC. miR-623 or miR-NC was transfected in (D) SGC-7901 and (E) BGC-823 cells with psiCHECK-WT-CCND1-3¢-UTR or psiCHECK-MUT-CCND1-3¢-UTR. Relative luciferase activity levels were measured at 48 h posttransfection. *p < 0.05 compared with miR-NC.

Article Snippet: In this study, mouse anti-human monoclonal CCND1 (1:1,000 dilution; Catalog No. sc-8396) and mouse antihuman monoclonal b-actin (1:1,000 dilution; Catalog No. sc-81178) primary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). b-Actin was utilized as a loading control for protein level normalization.

Techniques: Binding Assay, Quantitative RT-PCR, Western Blot, Transfection, Luciferase, Activity Assay

Journal: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

Article Title: MicroRNA-623 Targets Cyclin D1 to Inhibit Cell Proliferation and Enhance the Chemosensitivity of Cells to 5-Fluorouracil in Gastric Cancer

doi: 10.3727/096504018x15193469240508

Figure Lengend Snippet: Figure 4. CCND1 overexpression in GC tissues is inversely correlated with miR-623 level. (A) RT-qPCR and (B) Western blot were applied to measure the mRNA and protein expression levels of CCND1 in GC tissues and adjacent normal tissues, respectively. *p < 0.05 compared with normal tissues. (C) The association between CCND1 mRNA and miR-623 levels in GC tissues was assessed through Spearman’s correlation analysis. r = −0.5849, p = 0.0005.

Article Snippet: In this study, mouse anti-human monoclonal CCND1 (1:1,000 dilution; Catalog No. sc-8396) and mouse antihuman monoclonal b-actin (1:1,000 dilution; Catalog No. sc-81178) primary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). b-Actin was utilized as a loading control for protein level normalization.

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Expressing

Journal: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

Article Title: MicroRNA-623 Targets Cyclin D1 to Inhibit Cell Proliferation and Enhance the Chemosensitivity of Cells to 5-Fluorouracil in Gastric Cancer

doi: 10.3727/096504018x15193469240508

Figure Lengend Snippet: Figure 5. CCND1 overexpression reverses the effects of miR-623 on GC cells. (A) CCND1 protein expression was detected in SGC- 7901 and BGC-823 cells cotransfected with miR-623 mimic and pcDNA3.1 or pcDNA3.1-CCND1 through Western blot. *p < 0.05 compared with miR-NC. #p < 0.05 compared with miR-623 mimics + pDNA3.1-CCND1. CCK-8 assay (B), cell chemosensitivity assay (C), and flow cytometry analysis of cell apoptosis (D) were performed to determine cell proliferation, chemosensitivity to 5-FU, and apoptosis induced by 5-FU in differently treated SGC-7901 and BGC-823 cells, respectively. *p < 0.05 compared with miR-NC. #p < 0.05 compared with miR-623 mimics + pcDNA3.1-CCND1.

Article Snippet: In this study, mouse anti-human monoclonal CCND1 (1:1,000 dilution; Catalog No. sc-8396) and mouse antihuman monoclonal b-actin (1:1,000 dilution; Catalog No. sc-81178) primary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). b-Actin was utilized as a loading control for protein level normalization.

Techniques: Over Expression, Expressing, Western Blot, CCK-8 Assay, Flow Cytometry

Journal: Molecular medicine reports

Article Title: MicroRNA‑720 inhibits pancreatic cancer cell proliferation and invasion by directly targeting cyclin D1.

doi: 10.3892/mmr.2017.7732

Figure Lengend Snippet: Figure 3. CCND1 is a direct target of miR‑720 in pancreatic cancer. (A) Wild type and mutant of putative miR‑720 binding sites in the 3'‑untranslated region (3'‑UTR) of CCND1. (B) Relative luciferase activities in Panc‑1 and Sw1990 cells transfected with miR‑720 mimics or NC, together with pMIR‑CCND1‑3'‑UTR WT or pMIR‑CCND1‑3'‑UTR MUT. *P<0.05 compared with NC. (C and D) RT‑qPCR and Western blot analyses showed that miR‑720 overexpression decreased CCND1 mRNA and protein expression levels in Panc‑1 and Sw1990 cells. *P<0.05 compared with NC.

Article Snippet: Subsequently, the membranes were blocked by 5% non-fat milk in Tris-based saline-Tween 20 (TBST) for 1 h at room temperature and blotted with primary antibodies: mouse anti-human CCND1 monoclonal antibody (sc-450; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA) or mouse anti-human GAPDH monoclonal antibody (sc-47724; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA).

Techniques: Mutagenesis, Binding Assay, Luciferase, Transfection, Western Blot, Over Expression, Expressing

Journal: Molecular medicine reports

Article Title: MicroRNA‑720 inhibits pancreatic cancer cell proliferation and invasion by directly targeting cyclin D1.

doi: 10.3892/mmr.2017.7732

Figure Lengend Snippet: Figure 4. Inverse correlation between miR‑720 and CCND1 in pancreatic cancer tissues. (A and B) MRNA and protein expression levels of CCND1 in pancreatic cancer tissues and matched adjacent normal pancreatic tissues were determined using RT‑qPCR and Western blot analyses. *P<0.05 compared with adjacent normal pancreatic tissues. T, pancreatic cancer tissues; N, adjacent normal pancreatic tissues. (C) Evaluation of the inverse correlation between miR‑720 and CCND1 in pancreatic cancer tissues by Spearman's correlation analysis (r=‑0.6105, P=0.0020).

Article Snippet: Subsequently, the membranes were blocked by 5% non-fat milk in Tris-based saline-Tween 20 (TBST) for 1 h at room temperature and blotted with primary antibodies: mouse anti-human CCND1 monoclonal antibody (sc-450; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA) or mouse anti-human GAPDH monoclonal antibody (sc-47724; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA).

Techniques: Expressing, Western Blot

Journal: Molecular medicine reports

Article Title: MicroRNA‑720 inhibits pancreatic cancer cell proliferation and invasion by directly targeting cyclin D1.

doi: 10.3892/mmr.2017.7732

Figure Lengend Snippet: Figure 5. Upregulation of CCND1 prevents the inhibitory effects of miR‑720 on pancreatic cancer cells. (A) Protein expression of CCND1 in Panc‑1 and Sw1990 cells after transfection with miR‑720 mimics, NC or miR‑720 mimics, along with pcDNA 3.1‑CCND1. *P<0.05 compared with NC and miR‑720 mimics + pcDNA 3.1‑CCND1. (B and C) CCK8 assay and Matrigel invasion assay showed that CCND1 upregulation partly reversed the suppressive effects of miR‑720 on the proliferation and invasion of Panc‑1 and Sw1990 cells. *P<0.05 compared with NC and miR‑720 mimics + pcDNA 3.1‑CCND1.

Article Snippet: Subsequently, the membranes were blocked by 5% non-fat milk in Tris-based saline-Tween 20 (TBST) for 1 h at room temperature and blotted with primary antibodies: mouse anti-human CCND1 monoclonal antibody (sc-450; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA) or mouse anti-human GAPDH monoclonal antibody (sc-47724; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA).

Techniques: Expressing, Transfection, CCK-8 Assay, Invasion Assay

Journal: Cancer Cell International

Article Title: C-Phycocyanin exerts anti-cancer effects via the MAPK signaling pathway in MDA-MB-231 cells

doi: 10.1186/s12935-018-0511-5

Figure Lengend Snippet: Effects of C-phycocyanin on cell cycle distribution and the expressions of cyclins, CDKs, and CDK inhibitors in MDA-MB-231 cells. a C-Phycocyanin induced G0/G1 cell cycle arrest in MDA-MB-231 cells. Quantitative representation of cell cycle distribution after C-phycocyanin treatment for 24 h. b The expressions of Cyclin D1, Cyclin E, CDK2 and CDK4 were determined by western blot. c The expressions of p21 and p27 were determined by western blot

Article Snippet: Mouse anti-human COX-2, Cyclin D1, Cyclin E, CDK2, CDK4, p21, p27, Fas, cleaved-caspase 3, pro-caspase 3, ERK1/2, p-ERK1/2, JNK, p-JNK, p38 MAPK, p-p38 MAPK, AKT, and p-AKT monoclonal antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot

Journal: Cancer Cell International

Article Title: C-Phycocyanin exerts anti-cancer effects via the MAPK signaling pathway in MDA-MB-231 cells

doi: 10.1186/s12935-018-0511-5

Figure Lengend Snippet: Involvement of the MAPK pathway in C-phycocyanin-induced cell death. a Effect of C-phycocyanin on the PI3K/AKT signaling in MDA-MB-231 cells. After treatments, the expression levels of p-AKT and total AKT were analyzed by Western blotting. b Effect of C-phycocyanin on the MAPK signaling in MDA-MB-231 cells. After treatments, the levels of ERK1/2, JNK and p38 MAPK and their phosphorylated forms were analyzed by Western blotting. c The effects of inhibitors (SB203580 or SP600125) on cell viability were evaluated by CCK-8 assay. Significant difference compared with control group are indicated as *P < 0.05, **P < 0.005, and # P > 0.05. d The effects of PD98059 on the levels of COX-2, CDK2, CDK4, Cyclin D1, Cyclin E, p21 and p27 were evaluated by Western blotting

Article Snippet: Mouse anti-human COX-2, Cyclin D1, Cyclin E, CDK2, CDK4, p21, p27, Fas, cleaved-caspase 3, pro-caspase 3, ERK1/2, p-ERK1/2, JNK, p-JNK, p38 MAPK, p-p38 MAPK, AKT, and p-AKT monoclonal antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, CCK-8 Assay, Control